4D Reconstruction of the Beating Embryonic Heart From Two Orthogonal Sets of Parallel Optical Coherence Tomography Slice-Sequences

Sandeep Bhat, Irina V. Larina, Kirill V. Larin, Mary E. Dickinson, Michael Liebling

IEEE Transactions on Medical Imaging, Vol. 32, Issue 3, Mar, 2013

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Current methods to build dynamic optical coherence tomography (OCT) volumes of the beating embryonic heart involve synchronization of 2D+time slice-sequences acquired over separate heartbeats. Temporal registration of these sequences is performed either through gating or post-processing. While synchronization algorithms that exclusively rely on image-intrinsic signals allow forgoing external gating hardware, they are prone to error accumulation, require operator-supervised correction, or lead to non-isotropic resolution. Here, we propose an image based, retrospective reconstruction technique that uses two sets of parallel 2D+T slice-sequences, acquired perpendicularly to each other, to yield accurate and automatic reconstructions with isotropic resolution. The method utilizes the similarity of the data at the slice intersections to spatio-temporally register the two sets of slice sequences and fuse them into a high-resolution 4D volume. We characterize our method by using (1) simulated heart phantom datasets and (2) OCT datasets acquired from the beating heart of live cultured E9.5 mouse and E10.5 rat embryos. We demonstrate that while our method requires greater acquisition and reconstruction time compared to methods that use slices from a single direction, it produces more accurate and self-validating reconstructions since each set of reconstructed slices acts as a reference for the slices in the perpendicular set.


author={Sandeep Bhat and Irina V. Larina and Kirill V. Larin and Mary E. Dickinson and Michael Liebling},
journal={Medical Imaging, IEEE Transactions on},
title={4D Reconstruction of the Beating Embryonic Heart From Two Orthogonal Sets of Parallel Optical Coherence Tomography Slice-Sequences},

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