Motion-Based Structure Separation for Label-Free, High-Speed,
3D Cardiac Microscopy
Sandeep Bhat, Jungho Ohn, Michael Liebling
IEEE Transactions on Image Processing Vol. 21, Issue 8, August, 2012.
Download PDF | IEEE Xplore | View Movies
Capturing the dynamics of individual structures in the embryonic heart is an essential step for studying its function and development. Label-free brightfield (BF) microscopy allows for higher acquisition frame-rates than techniques requiring molecular labeling, without interfering with embryo viability or increasing equipment complexity. However, since different structures contribute similarly to image contrast, label-free microscopy lacks specificity. Here we mitigate this problem by separating a single-channel image series into multiple channels specific to the cardio-vascular structures, based only on their motion patterns. The technique is based on non-uniform temporal synchronizing, selecting, and combining images from multiple cardiac cycles and z-sections to produce 3D+time image volumes of one full cardiac cycle. The resulting data is highly suitable for velocity analysis and 3D-visualization. We characterize the separating capabilities of our technique on a synthetic cardiac dataset and demonstrate its practical applicability, by reconstructing a three-channel, 3D+time volumetric view of the heart in zebrafish embryos with an effective frame rate of 1000 volumes (256 x 256 x 20 voxels each) per second. This technique enables quantitative characterization of dynamic heart function during cardiogenesis.
@ARTICLE{bhat.liebling:1206,
author={Sandeep Bhat and Jungho Ohn and Michael Liebling},
journal={Image Processing, IEEE Transactions on},
title={Motion-Based Structure Separation for Label-Free High-Speed 3-D Cardiac Microscopy},
year={2012},
month={aug. },
volume={21},
number={8},
pages={3638 -3647}
}
Copyright © 2012 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from IEEE. This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.